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Anti-inflammatory and cytotoxic activities of Ipil-ipil (Leucaena leucocephala) leaf and seed extracts.


Krishia Marie Abayan,
Anthony J. Abella,
Mariel Grace Acain,
Arianne Janine Alo,
Hazel Baricar,
Ivana Mae Canlas,
Jana Danica Paraiso,
Genevieve Tan,
Maria Andrea Tenorio

Related Institution

Institute of Clinical Laboratory Sciences - Silliman University

Publication Information

Publication Type
Thesis Degree
Medical Technology
Publication Date
September 2013


Seed and leaf extracts from Leucaena leucocephala plants grown within the vicinity of Silliman University Farm were tested for its cytotoxic and anti-inflammatory activities at different concentration.  Ipil-ipil seeds and leaves were dried up before being crushed or pulverized in powdered form and then processed to form an aqueous solution.  Experimentation on the anti-inflammatory activity was done through the usage of albino rats as the test specimen.  Administration of the solution was done at a different route after controlled inflammation (albumin) was given and data was observed based on three criteria which are loss of function, swelling, and redness.  For the cytotoxic test, Artemia salina or brine shrimps were used as a test subjects.  A controlled number of the samples were made to live in a prepared environment brought up by different concentrations (5, 50, 500 ug/ml) of the extract in a test tube.  Results show that Leucaena leucocephala had no potent inhibitory effects for inflammation based on the three criteria considered by the group on the rat's paw, however, it did exhibit cytotoxicity by producing an LD50 effect in the 5 ug/ml concentration of the extract.


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